منابع مشابه
Nucleic acid reactivity and conformation. II. Reaction of cytosine and uracil with sodium bisulfite.
Treatment of calf thymus DNA with sodium bisulfite gave no deamination of cytosine to uracil, under conditions where 68 % of the cytosines of heat-denatured DNA were converted to uracil. This implies that the mutagenic properties of bisulfite are due to reaction with single-stranded DNA. Similarly, poly(1) . poly(C) was unreactive to bisulfite under conditions where poly(C) reacted readily. It ...
متن کاملHydrophilic Polymers of Ordered Conformation— Nucleic Acid Models
Nucleic acids contain severa' characteristic structural features: a chiral center adjacent to the base, a stereoregular, hydrophilic poly(phosphate-ribose) backbone which is negatively charged at neutral pH, and a helical secondary structure arising from conformational preferences enforced by base-stacking. We have prepared and studied several synthetic polynucleotide analogs having linear poly...
متن کاملNucleic acid reactivity: Challenges for next-generation semiempirical quantum models
Semiempirical quantum models are routinely used to study mechanisms of RNA catalysis and phosphoryl transfer reactions using combined quantum mechanical (QM)/molecular mechanical methods. Herein, we provide a broad assessment of the performance of existing semiempirical quantum models to describe nucleic acid structure and reactivity to quantify their limitations and guide the development of ne...
متن کاملRaman diagnosis of nucleic acid structure: sugar-puckering and glycosidic conformation in the guanosine moiety.
Observations of Raman spectra of various nucleic acids indicate that the guanine ring breathing frequency is sensitive to the internal rotation angle around the glycosidic bond and to the conformation of the five-membered ring of the ribose residue that is directly connected with the guanine residue in question. It is found that 682 cm-1 for C2'-endo-anti, at 665 cm-1 for C3'-endo-anti, and at ...
متن کاملA nonafluoro nucleoside as a sensitive 19f NMR probe of nucleic acid conformation.
A nucleoside carrying a perfluorinated tert-butyl group ( 4) was prepared by a Sonogashira coupling of 5-iodo-2'-deoxyuridine with 4,4,4-trifluoro-3,3-bis(trifluoromethyl)-1-butyne in nearly quantitative yield and subsequently incorporated into DNA oligomers. Thermal denaturation studies showed that 4 had a negligible effect on duplex stability when compared to thymidine. Transition from single...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43838-6